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Molecular characterization of the Tim9 homologue from the methylotrophic yeast Pichia methanolica
Author(s) -
Matsufuji Yoshimi,
Nakagawa Tomoyuki,
Ito Takashi,
Fujimura Shuki,
Chikui Mika,
Miyaji Tatsuro,
Tomizuka Noboru
Publication year - 2006
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1385
Subject(s) - biology , yeast , saccharomyces cerevisiae , pichia , biochemistry , amino acid , gene , peptide sequence , intron , cysteine , exon , genetics , microbiology and biotechnology , pichia pastoris , recombinant dna , enzyme
In this paper we describe molecular characterization of the TIM9 gene encoding the essential mitochondrial inner‐membrane protein in the methylotrophic yeast Pichia methanolica. PmTIM9 contains two exons corresponding to a gene product of 89 amino acid residues and a 140 bp intron. The deduced amino acid sequence exhibited high identity to those of other yeast Tim9ps, and possessed two CX 3 C motifs that contained two cysteine residues conserved among small Tim family proteins. Moreover, PmTIM9 had the ability to partially suppress the temperature sensitivity of Saccharomyces cerevisiae strain tim9‐3 , suggesting that PmTIM9 is a functional homologue of the ScTIM9 gene. Copyright © 2006 John Wiley & Sons, Ltd.