Molecular characterization of the  Tim9  homologue from the methylotrophic yeast  Pichia methanolica | Zendy

Matsufuji Yoshimi | Zendy; Nakagawa Tomoyuki | Zendy; Ito Takashi | Zendy; Fujimura Shuki | Zendy; Chikui Mika | Zendy; Miyaji Tatsuro | Zendy; Tomizuka Noboru | Zendy

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Molecular characterization of the Tim9 homologue from the methylotrophic yeast Pichia methanolica

Author(s) -

Matsufuji Yoshimi,

Nakagawa Tomoyuki,

Ito Takashi,

Fujimura Shuki,

Chikui Mika,

Miyaji Tatsuro,

Tomizuka Noboru

Publication year - 2006

Publication title -

yeast

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 102

eISSN - 1097-0061

pISSN - 0749-503X

DOI - 10.1002/yea.1385

Subject(s) - biology , yeast , saccharomyces cerevisiae , pichia , biochemistry , amino acid , gene , peptide sequence , intron , cysteine , exon , genetics , microbiology and biotechnology , pichia pastoris , recombinant dna , enzyme

In this paper we describe molecular characterization of the TIM9 gene encoding the essential mitochondrial inner‐membrane protein in the methylotrophic yeast Pichia methanolica. PmTIM9 contains two exons corresponding to a gene product of 89 amino acid residues and a 140 bp intron. The deduced amino acid sequence exhibited high identity to those of other yeast Tim9ps, and possessed two CX 3 C motifs that contained two cysteine residues conserved among small Tim family proteins. Moreover, PmTIM9 had the ability to partially suppress the temperature sensitivity of Saccharomyces cerevisiae strain tim9‐3 , suggesting that PmTIM9 is a functional homologue of the ScTIM9 gene. Copyright © 2006 John Wiley & Sons, Ltd.

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