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Isolation of Δ12 and ω3‐fatty acid desaturase genes from the yeast Kluyveromyces lactis and their heterologous expression to produce linoleic and α‐linolenic acids in Saccharomyces cerevisiae
Author(s) -
Kainou Kumiko,
Kamisaka Yasushi,
Kimura Kazuyoshi,
Uemura Hiroshi
Publication year - 2006
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1378
Subject(s) - kluyveromyces lactis , linoleic acid , biology , biochemistry , saccharomyces cerevisiae , fatty acid desaturase , linolenic acid , fatty acid , yeast , heterologous expression , oleic acid , polyunsaturated fatty acid , gene , recombinant dna
Two clones with homology to known fatty acid desaturase genes were isolated from the yeast Kluyveromyces lactis . The first gene, which we designate KlFAD2 , consists of 411 amino acids with an overall identity of 73.0% to FAD2 from Saccharomyces kluyveri . It exhibited Δ12 fatty acid desaturase activity when expressed in S. cerevisiae under the control of ADH1 promoter and produced endogenous linoleic acid. The second clone, which we designate KlFAD3 , consists of 415 amino acids with an overall identity of 79.3% to FAD3 from S. kluyveri . It exhibited ω3 fatty acid desaturase activity in S. cerevisiae when expressed under the control of ADH1 promoter in the presence of the exogenous substrate linoleic acid and produced α‐linolenic acid. Co‐expression of KlFAD2 and KlFAD3 resulted in the endogenous production of both linoleic and α‐linolenic acids. The yield of α‐linolenic acid reached 0.8% of total fatty acids and its production was not increased by adding exogenous oleic acid; α‐linolenic acid reached 8.7% when exogenous linoleic acid was available. Copyright © 2006 John Wiley & Sons, Ltd.