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A truncated derivative of nmt1 promoter exhibits temperature‐dependent induction of gene expression in Schizosaccharomyces pombe
Author(s) -
Kumar Raj,
Singh Jagmohan
Publication year - 2006
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1343
Subject(s) - schizosaccharomyces pombe , biology , gene expression , gene , schizosaccharomyces , derivative (finance) , genetics , expression (computer science) , microbiology and biotechnology , saccharomyces cerevisiae , financial economics , computer science , economics , programming language
Despite increasing exploitation of Schizosaccharomyces pombe as a model system there is a lack of convenient vectors for research and application. Expression with the commonly used promoter, nmt1 , requires a laborious regime involving the removal of repressor, thiamine, from a growing culture and further growth for 18 h to achieve maximum expression, thus underlining the need for more user‐friendly promoters. We report here the isolation and characterization of a truncated derivative of the nmt1 promoter having novel induction characteristics: it is induced by shift of growth temperature from 36 °C to 25 °C, achieving maximum expression within 3 h. Similar features of expression were observed with the reporter genes GFP and β‐galactosidase, a native gene, cdc18 , and a commercially important foreign therapeutic protein, streptokinase. The new promoter element offers additional advantages, such as lack of deleterious effect on cell viability and potential ability to express toxic proteins. These features make the new promoter a potentially better alternative to nmt1 , both as a research tool and for expression of commercially important proteins in Sz. pombe , and suggest the possibility of using similar approaches to design promoters with novel and useful properties. Copyright © 2006 John Wiley & Sons, Ltd.