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Phenotypes of yeast mutants lacking the mitochondrial protein Pet20p
Author(s) -
Polevoda Bogdan,
Panciera Yana,
Brown Steven P.,
Wei Jun,
Sherman Fred
Publication year - 2006
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1340
Subject(s) - biology , mitochondrion , saccharomyces cerevisiae , biochemistry , inner mitochondrial membrane , cytochrome c oxidase , protein subunit , yeast , staining , alternative oxidase , mutant , strain (injury) , microbiology and biotechnology , gene , genetics , anatomy
The pet20 ‐Δ deletion in Saccharomyces cerevisiae causes diminished growth on media containing non‐fermentable carbon sources when incubated at both above and below the 30 °C optimal growth temperature. Furthermore, the pet20 ‐Δ strain has a greatly reduced level of cytochrome c , especially at 37 °C. The pet20 ‐Δ strain was sensitive to high NaCl and CaCl 2 concentrations, hydrogen peroxide, oligomycin, polymixin B, amphotericin B and fluconazole. Biochemical fractionation and immunofluorescence staining demonstrated that Pet20p is located primarily in the mitochondria. Rhodamine B staining of pet20 ‐Δ cells showed an altered mitochondrial staining, indicating the possible lack of the mitochondrial membrane potential. We suggest that PET20 encodes a protein required for proper assembly or maintenance of mitochondrial components, but does not serve an enzymatic role. It is also possible that Pet20p may constitute a non‐catalytic subunit of an uncharacterized mitochondrial complex or serve as a transporter or a coupling factor. Copyright © 2006 John Wiley & Sons, Ltd.