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Cloning and functional analysis of the orotidine‐5′‐phosphate decarboxylase gene ( PbrURA3 ) of the pathogenic fungus Paracoccidioides brasiliensis
Author(s) -
Reinoso Cristina,
Sorais Françoise,
NiñoVega Gustavo A.,
Fermiñán Encarnación,
SanBlas Gioconda,
Domínguez Angel
Publication year - 2005
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1248
Subject(s) - paracoccidioides brasiliensis , biology , fungus , microbiology and biotechnology , cloning (programming) , paracoccidioides , gene , pathogenic fungus , molecular cloning , paracoccidioidomycosis , genetics , botany , gene expression , computer science , programming language
A genomic clone encoding the Paracoccidioides brasiliensis orotidine monophosphate decarboxylase gene ( PbrURA3 ) was isolated by screening a subgenomic plasmid DNA library of this fungus, using a PCR amplification product of the gene as a probe. Sequence analysis revealed that the gene contains an open reading frame of 855 bp with a single intron (162 bp), and encodes a putative 285 amino acids polypeptide of estimated molecular weight 31.1 kDa and isoelectric point 6.5. The deduced amino acid sequence predicted a 73.4% identity with orotidine monophosphate decarboxylase of Aspergillus nidulans . Functionality of the gene was demonstrated by transformation into a Saccharomyces cerevisiae ura3 null mutant. The PbrURA3 gene sequence has been submitted to GenBank database under Accession No. AJ133782. Copyright © 2005 John Wiley & Sons, Ltd.