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Cloning, sequencing and application of the LEU2 gene from the sour dough yeast Candida milleri
Author(s) -
Turakainen Hilkka,
Korhola Matti
Publication year - 2005
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1246
Subject(s) - biology , genetics , nucleic acid sequence , gene , saccharomyces cerevisiae , kluyveromyces lactis , molecular cloning , restriction enzyme , coding region , microbiology and biotechnology , peptide sequence
Abstract We have cloned by complementation in Saccharomyces cerevisiae and sequenced a LEU2 gene from the sour dough yeast Candida milleri CBS 8195 and studied its chromosomal location. The LEU2 coding sequence was 1092 nt long encoding a putative β‐isopropylmalate dehydrogenase protein of 363 amino acids. The nucleotide sequence in the coding region had 71.6% identity to S. cerevisiae LEU2 sequence. On the protein level, the identity of C. milleri Leu2p to S. cerevisiae Leu2p was 84.1%. The DNA sequence of the C. milleri LEU2 gene was submitted to GenBank and assigned Accession No. AY571337. The CmLEU2 DNA probe hybridized to one to three chromosomal bands and two or three Bam HI restriction fragments in C. milleri but did not give any signal to chromosomes or restriction fragments of C. albicans, S. cerevisiae, S. exiguus or Torulaspora delbrueckii . Using CmLEU2 probe for DNA hybridization makes it easy to quickly identify C. milleri among other sour dough yeasts. Copyright © 2005 John Wiley & Sons, Ltd.