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Importance of cell wall mannoproteins for septum formation in Saccharomyces cerevisiae
Author(s) -
Schmidt Martin,
Strenk Meghan E.,
Boyer Michael P.,
Fritsch Bryan J.
Publication year - 2005
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1242
Subject(s) - saccharomyces cerevisiae , biology , cytokinesis , mutant , cell wall , septin , glycosylation , cell division , microbiology and biotechnology , yeast , biochemistry , glucan , cell , gene
The mannosyltransferase mutants mnn9 and mnn10 were isolated in a genetic screen for septation defects in Saccharomyces cerevisiae. Ultrastructural examination of mutant cell walls revealed markedly thin septal structures and occasional failure to construct trilaminar septa, which then led to the formation of bulky default septa at the bud neck. In the absence of a functional septation apparatus, mnn10 mutants are unable to complete cytokinesis and die as cell chains with incompletely separated cytoplasms, indicating that mannosylation defects impair the ability to form remedial septa. We could not detect N‐linked glycosylation of the β(1,3)glucan synthase Fks1p and mnn10 defects do not change the molecular weight or abundance of the protein. We discuss a model explaining the pleiotropic effects of impaired N‐linked protein glycosylation on septation in S. cerevisiae . Copyright © 2005 John Wiley & Sons, Ltd.