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Doxycycline, the drug used to control the tet ‐regulatable promoter system, has no effect on global gene expression in Saccharomyces cerevisiae
Author(s) -
Wishart Jill A.,
Hayes Andrew,
Wardleworth Leanne,
Zhang Nianshu,
Oliver Stephen G.
Publication year - 2005
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1225
Subject(s) - doxycycline , biology , gene , saccharomyces cerevisiae , transcription (linguistics) , transcriptome , phenotype , genetics , yeast , promoter , gene expression , computational biology , antibiotics , linguistics , philosophy
The tet ‐regulatable promoter system is commonly used for genetic studies in many eukaryotic organisms. The promoter is regulated using doxycycline. There are no obvious phenotypic effects observed when doxycycline is added to the growth medium of yeast to control expression from the promoter. It is widely accepted that doxycycline is innocuous to yeast. Global genetic studies are now commonplace and the tetO ‐system is being used in transcriptome studies. Hence, we wanted to ensure that the absence of phenotypic effects, on addition of doxycycline to the growth medium, is mirrored in transcriptome data. We have demonstrated that doxycycline has no significant effect on global transcription levels and will continue to use the tetO ‐regulatable promoter system for genetic studies. Copyright © 2005 John Wiley & Sons, Ltd.