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Involvement of actin and polarisome in morphological change during spore germination of Saccharomyces cerevisiae
Author(s) -
Kono Keiko,
Matsunaga Rino,
Hirata Aiko,
Suzuki Genjiro,
Abe Mitsuhiro,
Ohya Yoshikazu
Publication year - 2005
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1205
Subject(s) - biology , germination , actin , saccharomyces cerevisiae , depolarization , morphogenesis , microbiology and biotechnology , spore germination , botany , yeast , biophysics , genetics , gene
Abstract We studied the morphological changes of Saccharomyces cerevisiae ascospores during germination. Initiation of germination is followed by polarization of actin patches, maintaining their localization to the site of cell surface growth. Loss of polarisome components, Spa2p, Pea2p, Bud6p or Bni1p, results in depolarization of actin patches. Green fluorescent protein‐fused polarisome components exhibit the polarized localization, implying that polarisome is involved in the polarized outgrowth during germination. At the late stage of germination, we found that actin patches temporally depolarize before bud emergence. The observation that loss of Cla4p extends the polarized growth period suggests that Cla4p is involved in the actin‐depolization step. Actin polarization in the initial stage is accelerated by overexpression of Ras2p, whereas hyperpolarization is continuously observed by overexpression of Rho1p. Thus, yeast spore germination is a morphological event that is regulated by a number of factors implicated in mitotic bud morphogenesis. Copyright © 2005 John Wiley & Sons, Ltd.