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Pro‐oxidant action of phloxine B on fission yeast Schizosaccharomyces pombe
Author(s) -
Mutoh Norihiro,
Kawabata Masao,
Nakagawa Chiaki W.,
Kitajima Satoko
Publication year - 2005
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1196
Subject(s) - schizosaccharomyces pombe , biology , yeast , fission , schizosaccharomyces , microbiology and biotechnology , botany , biochemistry , saccharomyces cerevisiae , physics , nuclear physics , neutron
A Schizosaccharomyces pombe mutant deficient in Cu,Zn‐superoxide dismutase ( sod1 mutant) was hypersensitive to phloxine B, which is used as a food‐colouring agent and also to distinguish diploid strains of Sz. pombe from haploid strains, under illumination with light. The pro‐oxidant nature of phloxine B was confirmed biochemically. The carbonyl content of proteins (which represents protein oxidation) increased, and the reduced form of glutathione was transiently decreased by phloxine B treatment under illumination with light. When cells were treated with phloxine B under light, carbonyl content of proteins in the sod1 mutant was greater than that in the wild‐type and amount of glutathione was much decreased in the sod1 mutant compared with the wild‐type. Genes induced by oxidative stress were induced by phloxine B under illumination with light and some were induced by phloxine B without light. Copyright © 2005 John Wiley & Sons, Ltd.