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Analysis of the meiotic role of the mitochondrial ribosomal proteins Mrps17 and Mrpl37 in Saccharomyces cerevisiae
Author(s) -
Hanlon Sean E.,
Xu Zhiheng,
Norris David N.,
Vershon Andrew K.
Publication year - 2004
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1174
Subject(s) - biology , saccharomyces cerevisiae , ribosomal protein , gene , genetics , ribosomal rna , homology (biology) , mitochondrial dna , complementary dna , microbiology and biotechnology , ribosome , rna
Sporulation in the yeast Saccharomyces cerevisiae is a complex and tightly regulated pathway that involves the induction of a large number of genes. We have identified MRPS17 in a cDNA library enriched for sporulation‐specific genes. Homology searches show that the first one‐third of Mrps17 has strong sequence similarity to bacterial S17 proteins, suggesting that Mrps17 is a potential mitochondrial ribosomal protein. This is further supported by the fact that mrps17 Δ cells are respiratory‐deficient and that a Mrps17–GFP fusion localizes to the mitochondria. We have confirmed by Northern blot analysis that both MRPS17 and MRPL37 are strongly induced during the middle stages of sporulation and that this induction is dependent on the presence of a m iddle s porulation e lement (MSE) in the promoters of these genes. Interestingly, we found that Mrps17 and Mrpl37, but not other mitochondrial ribosomal proteins, accumulate during the middle stages of sporulation. These results suggest that Mrps17 and Mrpl37 may have additional meiosis‐specific roles. Copyright © 2004 John Wiley & Sons, Ltd.