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Interrelationships among Atg proteins during autophagy in Saccharomyces cerevisiae
Author(s) -
Suzuki Kuninori,
Noda Takeshi,
Ohsumi Yoshinori
Publication year - 2004
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1152
Subject(s) - autophagy , biology , autophagosome , saccharomyces cerevisiae , microbiology and biotechnology , mutant , green fluorescent protein , gene , biochemistry , apoptosis
Macroautophagy is a bulk degradation process induced by nutrient starvation in eukaryotic cells. In Saccharomyces cerevisiae , 16 ATG genes are essential for autophagosome formation. Recently, we demonstrated that these ATG genes can be classified into three groups on the basis of localization of GFP–Atg8p/Aut7p and Atg5p/Apg5p–GFP under nutrient‐rich conditions. In this study, we analysed the intracellular localization of GFP–Atg8p, an autophagosome marker, in atg mutants under autophagy‐inducing conditions; the localization of GFP–Atg8p exhibited several distinct patterns. Subsequent analyses revealed epistatic interrelationships among Atg proteins during the process of autophagosome formation. Copyright © 2004 John Wiley & Sons, Ltd.