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Cell shape and growth of budding yeast cells in restrictive microenvironments
Author(s) -
Suzuki Masaya,
Asada Yosuke,
Watanabe Daisuke,
Ohya Yoshikazu
Publication year - 2004
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1132
Subject(s) - biology , microbiology and biotechnology , saccharomyces cerevisiae , yeast , budding , cell division , morphogenesis , budding yeast , cell cycle , cell , cell growth , genetics , gene
Effects of limited growth space on the cell morphology and cell growth are investigated by creating rigid outside environments. The cube‐shaped holes big enough for a single cell of the budding yeast Saccharomyces cerevisiae were prepared with a focused ion‐beam (FIB), commonly used for processing semiconductors. We demonstrated that the outline of the cells changes their ellipsoidal morphology into a cubic form when the daughter cells are grown in the holes, indicating that yeast cells change their shape in response to external limited space. The yeast cells grown in the microenvironments exhibit neither bud formation nor nuclear division. Although restricted growth caused by the physical barriers leads to the block of cell cycle progression in the wild‐type cells, swe 1Δ cells defective in the morphogenesis checkpoint become binucleate after being grown in the microenvironments. These results suggest that yeast cells under spatial restriction arrest cell cycle progression in a Swelp‐dependent manner. Copyright © 2004 John Wiley & Sons, Ltd.