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The KlSRB10 gene from Kluyveromyces lactis
Author(s) -
Núñez Laura,
FernándezOtero Cristina,
RodríguezBelmonte Esther,
Cerdán M. Esperanza
Publication year - 2004
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1117
Subject(s) - kluyveromyces lactis , biology , kluyveromyces , gene , genetics , computational biology , saccharomyces cerevisiae
We report the cloning and sequencing of a gene from Kluyveromyces lactis with high homology to the SRB10 gene (alias UME5, SSN3, GIG2, NUT7, RYE5 ) from Saccharomyces cerevisiae and other organisms. The KlSRB10 gene is located in a similar configuration to that found in S. cerevisiae , flanked by NOT4 and a gene with high similarity to YPL041c. The translated protein contains 593 amino acids and the characteristic domains of kinases from the CMGC subgroup. The functional relationship to yeast SRB10 is demonstrated by complementation of mutant phenotypes in a haploid S. cerevisiae strain containing a null allele. Submitted to EMBL data library under Accession No. AJ532841. Copyright © 2004 John Wiley & Sons, Ltd.