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Isolation of a GPD gene from Debaryomyces hansenii encoding a glycerol 3‐phosphate dehydrogenase ( NAD + )
Author(s) -
Thomé Patricia E.
Publication year - 2003
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1070
Subject(s) - biology , debaryomyces hansenii , dehydrogenase , gene , nad+ kinase , glycerol 3 phosphate dehydrogenase , biochemistry , glycerol , phosphate , isolation (microbiology) , microbiology and biotechnology , genetics , enzyme , yeast
A gene homologous to GPD1, coding for glycerol‐3‐phosphate dehydrogenase (sn‐glycerol 3‐phosphate: NAD + oxidoreductase, EC 1.1.1.8), has been isolated from the halophilic yeast Debaryomyces hansenii by complementation of a Saccharomyces cerevisiae gpd1 Δ mutant. DNA sequencing of the complementing genomic clone indicated the existence of an open reading frame encoding a protein with 369 amino acids. Comparative analysis of the deduced amino acid sequence showed high similarity to homologous genes described for other eukaryotic GPD enzymes. The sequence has been submitted to the GenBank database under Accession No. AY333427. Copyright © 2003 John Wiley & Sons, Ltd.