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Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae
Author(s) -
Paiva Sandra,
Devaux Frederic,
Barbosa Sonia,
Jacq Claude,
Casal Margarida
Publication year - 2004
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1056
Subject(s) - saccharomyces cerevisiae , yarrowia , biology , yeast , biochemistry , acetic acid , permease , gene expression , gene , gene cluster , transporter
To identify new genes involved in acetate uptake in Saccharomyces cerevisiae , an analysis of the gene expression profiles of cells shifted from glucose to acetic acid was performed. The gene expression reprogramming of yeast adapting to a poor non‐fermentable carbon source was observed, including dramatic metabolic changes, global activation of translation machinery, mitochondria biogenesis and the induction of known or putative transporters. Among them, the gene ADY2/YCR010c was identified as a new key element for acetate transport, being homologous to the Yarrowia lipolytica GPR1 gene, which has a role in acetic acid sensitivity. Disruption of ADY2 in S. cerevisiae abolished the active transport of acetate. Microarray analyses of ady2 Δ strains showed that this gene is not a critical regulator of acetate response and that its role is directly connected to acetate transport. Ady2p is predicted to be a membrane protein and is a valuable acetate transporter candidate. Copyright © 2004 John Wiley & Sons, Ltd.