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Role of Tea1p, Tea3p and Pom1p in the determination of cell ends in Schizosaccharomyces pombe
Author(s) -
Niccoli Teresa,
Arellano Manuel,
Nurse Paul
Publication year - 2003
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1054
Subject(s) - schizosaccharomyces pombe , biology , microtubule , microbiology and biotechnology , schizosaccharomyces , cytoskeleton , moesin , ezrin , cell , actin , cell growth , cell polarity , yeast , saccharomyces cerevisiae , genetics
Schizosaccharomyces pombe cells are rod‐shaped and grow along a single axis from their two ends. Microtubules extend from the cell centre terminating at the cell ends. The ERM(ezrin/radixin/moesin)‐like proteins Tea1p and Tea3p, and the Dyrk‐like kinase Pom1p are cell end markers involved in the regulation of growth and microtubular dynamics at the cell ends. We have analysed the relative contribution of these three proteins to the determination of cell ends as sites both for cell growth and for microtubular termination. Pom 1Δ, in combination with Tea 1Δ or Tea 3Δ, has the greatest difficulty in relocalizing actin to the cell ends following actin depolymerization and generates the most defective growth pattern. Tea 1Δ, in combination with Pom 1Δ or Tea 3Δ, displays the highest number of microtubules bending round the cell ends. Tea 1Δ Pom 1Δ, which has the most defective growth pattern and microtubules, also displays the highest number of branched cells. We show that Tea1p, Tea3p and Pom1p all contribute, to different extents, to the determination of cell ends, as sites for both cell growth and microtubular termination. We also show that the fission yeast cell relies on both the positioning of landmarks and a properly organized microtubule cytoskeleton to direct cell growth. Copyright © 2003 John Wiley & Sons, Ltd.