Premium
Protein A‐tagging for purification of native macromolecular complexes from Candida albicans
Author(s) -
Blackwell Chris,
Russell Claire L.,
Argimon Silvia,
Brown Alistair J. P.,
Brown Jeremy D.
Publication year - 2003
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1036
Subject(s) - candida albicans , biology , computational biology , open reading frame , toolbox , biochemistry , peptide sequence , genetics , programming language , gene , computer science
Protein A‐tagging has become an important tool in characterization of protein–protein interactions in many systems, allowing purification of multicomponent complexes under native conditions. Here we provide a set of vectors that allow protein A‐tagging in Candida albicans , through addition of the tag to open reading frames. These vectors were successfully used to generate stably tagged proteins that were functional, shown to be localized appropriately or assembled into complexes. These new vectors comprise a useful addition to the C. albicans molecular toolbox. Copyright © 2003 John Wiley & Sons, Ltd.