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Distribution and sequence analysis of a novel Ty3‐like element in natural Saccharomyces paradoxus isolates
Author(s) -
Fingerman Ethan G.,
Dombrowski Peter G.,
Francis Chantal A.,
Sniegowski Paul D.
Publication year - 2003
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1005
Subject(s) - biology , transposable element , population , genbank , saccharomyces cerevisiae , genetics , genome , yeast , gene , demography , sociology
Little is known about the transposable elements of species closely related to Saccharomyces cerevisiae . We present a novel transposable element in Saccharomyces paradoxus , a close congener of S. cerevisiae . Sequence analysis of this element, designated Ty3‐1p, indicates that it is a homologue of the S. cerevisiae Ty3 element. Ty3‐1p shares 82% nucleotide identity with an S. cerevisiae Ty3 element and appears to be structured identically to Ty3, containing two overlapping open reading frames, six retroviral‐like domains, a J domain, and flanking sigma‐like elements. A sigma element from Ty3‐1p is 75% identical to a Ty3 sigma element. There is no evidence of horizontal transfer of Ty3 in Saccharomyces sensu stricto . We assess the distributions of Ty3p and Ty3 element insertions in natural population samples of S. paradoxus and S. cerevisiae . The S. paradoxus population sample exhibits Ty3p insertions present at a variety of sites at low frequency; this suggests that Ty3p elements are active in the sampled population. The S. cerevisiae population sample exhibits a uniform Ty3 hybridization profile in which all element insertions appear to be fixed. We comment on the possible causes of these contrasting observed distributions (GenBank Accession Nos AY198186 and AY198187). Copyright © 2003 John Wiley & Sons, Ltd.