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SIR ‐dependent repression of non‐telomeric genes in Saccharomyces cerevisiae ?
Author(s) -
Marchfelder Uta,
Rateitschak Katja,
EhrenhoferMurray Ann E.
Publication year - 2003
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1001
Subject(s) - biology , psychological repression , gene , saccharomyces cerevisiae , telomere , genetics , transcription (linguistics) , repressor , regulation of gene expression , gene expression , linguistics , philosophy
The proteins Sir2, Sir3 and Sir4 repress transcription of the silent mating‐type loci HML and HMR and of reporter genes inserted at telomeres. Previous microarray analyses suggested that additional non‐telomeric genes exist which are repressed by the Sir proteins. In this study, we tested the expression of 12 such genes by Northern analysis and RT–PCR. However, we were unable to verify their SIR ‐dependent regulation, which suggests that SIR ‐mediated repression may be restricted to the known repressed regions. Copyright © 2003 John Wiley & Sons, Ltd.