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Direct determination of mineral nutrients in soybean leaves under vivo conditions by portable X‐ray fluorescence spectroscopy
Author(s) -
Tadeu Costa Junior Geovani,
Nunes Lidiane Cristina,
Feresin Gomes Marcos Henrique,
Almeida Eduardo,
Pereira de Carvalho Hudson Wallace
Publication year - 2019
Publication title -
x‐ray spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.447
H-Index - 45
eISSN - 1097-4539
pISSN - 0049-8246
DOI - 10.1002/xrs.3111
Subject(s) - chemistry , analytical chemistry (journal) , x ray fluorescence , inductively coupled plasma atomic emission spectroscopy , micronutrient , inductively coupled plasma , spectroscopy , nutrient , fluorescence spectrometry , filter paper , fluorescence spectroscopy , fluorescence , chromatography , plasma , physics , organic chemistry , quantum mechanics
A portable energy dispersive X‐ray fluorescence (XRF) spectrometer furnished with an Rh X‐ray tube was evaluated for the determination of macronutrients and micronutrients in soybean leaves ( Glycine max L.). XRF instrumental parameters were optimized in a univariate way, and emission intensities were measured for 60 s and under vacuum for macronutrients, and during 180 s, under air, and 305 μm Al/25.4 μm Ti filter, for micronutrients. Fresh and dried leaves were irradiated, and it was possible to identify P, K, Ca, S, Mn, Fe, Cu, and Zn Kα emission lines. For comparative purpose, the samples were also microwave assisted, digested and analyzed by inductively coupled plasma optical emission spectrometry. In general, linear correlations between K, Ca, Mn, Fe, Cu, and Zn concentrations in the tested samples and the corresponding portable XRF (pXRF) intensities were obtained. The linear correlation coefficients ( R 2 ) ranged from 0.42 to 0.86. In addition, the detection limits were suitable for plant nutrient diagnosis. It is demonstrated that pXRF is a simple and powerful tool for analysis of plant materials.