Premium
Increased zinc accumulation in mineralized osteosarcoma tissue measured by confocal synchrotron radiation micro X‐ray fluorescence analysis
Author(s) -
Rauwolf Mirjam,
Pemmer Bernhard,
Roschger Andreas,
Turyanskaya Anna,
Smolek Stephan,
Maderitsch Angelika,
Hischenhuber Peter,
Foelser Martin,
Simon Rolf,
Lang Susanna,
Puchner Stephan E.,
Windhager Reinhard,
Klaushofer Klaus,
Wobrauschek Peter,
Hofstaetter Jochen G.,
Roschger Paul,
Streli Christina
Publication year - 2016
Publication title -
x‐ray spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.447
H-Index - 45
eISSN - 1097-4539
pISSN - 0049-8246
DOI - 10.1002/xrs.2727
Subject(s) - osteosarcoma , zinc , chemistry , grading (engineering) , confocal , radiation therapy , pathology , nuclear medicine , soft tissue , synchrotron radiation , medicine , radiology , biology , optics , physics , ecology , organic chemistry
Abnormal tissue levels of certain trace elements such as zinc (Zn) were reported in various types of cancer. Little is known about the role of Zn in osteosarcoma. Using confocal synchrotron radiation micro X‐ray fluorescence analysis, we characterized the spatial distribution of Zn in high‐grade sclerosing osteosarcoma of nine patients (four women/five men; seven knee/one humerus/one femur) following chemotherapy and wide surgical resection. Levels were compared with adjacent normal tissue. Quantitative backscattered electron imaging as well as histological examinations was also performed. On average, the ratio of medians of Zn count rates (normalized to calcium) in mineralized tumor tissue was about six times higher than in normal tissue. There was no difference in Zn levels between tumor fraction areas with a low fraction and a high fraction of mineralized tissue, which were clearly depicted using quantitative backscattered electron imaging. Moreover, we found no correlation between the Zn values and the type of tumor regression according to the Salzer‐Kuntschik grading. The underlying mechanism of Zn accumulation remains unclear. Given the emerging data on the role of trace elements in other types of cancer, our novel results warrant further studies on the role of trace elements in bone cancer. Copyright © 2016 The Authors. X‐Ray Spectrometry published by John Wiley & Sons Ltd.