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Determination of low Z in human serum of patients with Idiopathic Thrombocytopenic Purpura by total reflection X‐ray fluorescence
Author(s) -
Canellas C. G. L.,
Carvalho S. M. F.,
Bellido A. V. B.,
Leitão R. G.,
Anjos M. J.,
Lopes R. T.
Publication year - 2013
Publication title -
x‐ray spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.447
H-Index - 45
eISSN - 1097-4539
pISSN - 0049-8246
DOI - 10.1002/xrs.2466
Subject(s) - thrombocytopenic purpura , chlorine , platelet , chemistry , fluorescence , hemoglobin , medicine , gastroenterology , nuclear medicine , analytical chemistry (journal) , nuclear chemistry , chromatography , physics , organic chemistry , quantum mechanics
Idiopathic Thrombocytopenic Purpura (ITP) is a blood disorder characterized by an abnormal decrease in the number of platelets in the blood. In this work, 40 patients suffering from ITP and 60 healthy volunteers (Control Group) were analyzed. All the serum samples were collected from people who live in the urban area of Rio de Janeiro City/Brazil. The measurements were performed at the X‐ray fluorescence Beamline at the Brazilian National Synchrotron Light Laboratory, in Campinas, São Paulo using a monochromatic beam with maximum energy of 20 keV for the excitation and an Ultra‐LEGe detector with resolution of 148 eV at 5.9 keV. Standard solutions with Vanadium as internal standard were prepared for the calibration system. It was possible to determine the elemental concentrations of the following six elements: Na, P, S, Cl, K and Ca. The Student's t ‐test was used to analyze significant differences (α = 0.05) between patients with ITP and Control Group. The elements that presented significant differences for the mean of their concentrations between each one from the ITP group and Control Group in µg g −1 , were phosphorous (136 ± 12 and 92 ± 12), sulfur (1077 ± 97 and 847 ± 80), chlorine (2905 ± 385 and 2266 ± 378), potassium (137 ± 118 and 82 ± 15) and calcium (64 ± 7 and 44 ± 6), respectively. Copyright © 2013 John Wiley & Sons, Ltd.