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Determination of cobalt marker in cow ruminal fluid by EDXRF and SRTXRF
Author(s) -
de Almeida Eduardo,
Rogério Massoni Paulo,
Menegário Amauri Antonio,
Leite Laudi Cunha,
Duarte Lanna Dante Pazzanese,
do Nascimento Filho Virgílio Franco
Publication year - 2011
Publication title -
x‐ray spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.447
H-Index - 45
eISSN - 1097-4539
pISSN - 0049-8246
DOI - 10.1002/xrs.1361
Subject(s) - x ray fluorescence , analytical chemistry (journal) , materials science , detection limit , fluorescence , chromatography , synchrotron radiation , synchrotron , sample (material) , chemistry , optics , physics
This article describes a comparison of conventional energy‐dispersive X‐ray fluorescence (EDXRF) and synchrotron radiation total‐reflection X‐ray fluorescence (SRTXRF) for Co determination in ruminal fluid from Holstein cow. This element is used as marker for animal nutrition studies. For EDXRF, 200 µl of the sample were dried on 6.35 µm Mylar film at 60 °C. The excitation was carried out using an X‐ray tube with Mo target and Zr filter operated at 30 kV/20 mA. For SRTXRF, 10 µl of the sample were pipetted on a Lucite carrier and dried at 60 °C. In both the techniques, Ga was used as internal standard and the acquisition time was 200 s. The trueness of both techniques was evaluated through the standard addition method, the recoveries obtained by SRTXRF and EDXRF were 76 and 99%, and the limits of detection, 13 and 240 µg l −1 , respectively. Copyright © 2011 John Wiley & Sons, Ltd.