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PIXE and micro‐PIXE studies of exogenous element distributions in animal organs
Author(s) -
Munnik F.,
Mutsaers P. H. A.,
Rokita E.,
de Voigt M. J. A.
Publication year - 1991
Publication title -
x‐ray spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.447
H-Index - 45
eISSN - 1097-4539
pISSN - 0049-8246
DOI - 10.1002/xrs.1300200607
Subject(s) - strontium , embryo , chemistry , gestation , radiochemistry , zoology , nuclear chemistry , anatomy , biology , pregnancy , genetics , organic chemistry , microbiology and biotechnology
A pilot study was undertaken with aluminium and strontium in order to determine the path taken when they enter an organism. Rabbits received an intramuscular injection of A1, and thin tissue cryo‐sections and pelletized and incinerated samples were investigated by the PIXE method. The best PIXE detection limits ( ca. 60 μg g −1 dry weight) were obtained for thin tissue samples. On the basis of the chemical similarity of Ca and Sr, Sr ions were used to mark the Ca incorporation in the mineralized tissue. At different times of gestation (20–30 d), pregnant rabbits received an intraperitoneal injection of Sr. The animals were killed 7 h after the injection and the hind legs and skulls of the embryos were subjected directly to micro‐PIXE measurements. The results revealed that Sr incorporation in the embryo is an age‐dependent process. The highest Sr concentrations were found for 24‐d old embryos whereas at the end of gestation (30 d) the Sr concentration had decreased by a factor of ca. 10.