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Pre‐mRNA 3′‐end processing complex assembly and function
Author(s) -
Chan Serena,
Choi EunA,
Shi Yongsheng
Publication year - 2010
Publication title -
wiley interdisciplinary reviews: rna
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.225
H-Index - 71
eISSN - 1757-7012
pISSN - 1757-7004
DOI - 10.1002/wrna.54
Subject(s) - rna processing , polyadenylation , messenger rna , precursor mrna , rna , directionality , cleavage (geology) , rna binding protein , microbiology and biotechnology , computational biology , biology , computer science , genetics , rna splicing , gene , paleontology , fracture (geology)
The 3′‐ends of almost all eukaryotic mRNAs are formed in a two‐step process, an endonucleolytic cleavage followed by polyadenylation (the addition of a poly‐adenosine or poly(A) tail). These reactions take place in the pre‐mRNA 3′ processing complex, a macromolecular machinery that consists of more than 20 proteins. A general framework for how the pre‐mRNA 3′ processing complex assembles and functions has emerged from extensive studies over the past several decades using biochemical, genetic, computational, and structural approaches. In this article, we review what we have learned about this important cellular machine and discuss the remaining questions and future challenges. WIREs RNA 2011 2 321–335 DOI: 10.1002/wrna.54 This article is categorized under: RNA Processing > 3' End Processing