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Practical guide for circular RNA analysis: Steps, tips, and resources
Author(s) -
Tsitsipatis Dimitrios,
Gorospe Myriam
Publication year - 2020
Publication title -
wiley interdisciplinary reviews: rna
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.225
H-Index - 71
eISSN - 1757-7012
pISSN - 1757-7004
DOI - 10.1002/wrna.1633
Subject(s) - computational biology , microrna , rna , circular rna , riboswitch , biology , non coding rna , rna interference , genetics , gene
Recent technological advances in RNA sequencing and analysis have allowed an increasingly thorough investigation of a previously unexplored class of transcripts, circular (circ)RNAs. Accumulating evidence suggests that circRNAs have unique functions which often rely on their association with microRNAs and RNA‐binding proteins. Through these interactions, circRNAs have been implicated in major cellular processes and hence in the pathophysiology of a range of diseases. Here, we provide guidelines to consider when developing studies on circRNAs, including detecting and selecting the circRNAs, identifying their binding partners and sites of interaction, modulating circRNA levels, assessing copy numbers and stoichiometry, and addressing other points unique to circRNA analysis. This article is categorized under: Regulatory RNAs/RNAi/Riboswitches > Regulatory RNAs