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Approaches for measuring the dynamics of RNA–protein interactions
Author(s) -
Licatalosi Donny D.,
Ye Xuan,
Jankowsky Eckhard
Publication year - 2019
Publication title -
wiley interdisciplinary reviews: rna
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.225
H-Index - 71
eISSN - 1757-7012
pISSN - 1757-7004
DOI - 10.1002/wrna.1565
Subject(s) - rna , computational biology , riboswitch , biology , non coding rna , gene expression , rna binding protein , microbiology and biotechnology , genetics , gene
RNA–protein interactions are pivotal for the regulation of gene expression from bacteria to human. RNA–protein interactions are dynamic; they change over biologically relevant timescales. Understanding the regulation of gene expression at the RNA level therefore requires knowledge of the dynamics of RNA–protein interactions. Here, we discuss the main experimental approaches to measure dynamic aspects of RNA–protein interactions. We cover techniques that assess dynamics of cellular RNA–protein interactions that accompany biological processes over timescales of hours or longer and techniques measuring the kinetic dynamics of RNA–protein interactions in vitro. This article is categorized under: RNA Interactions with Proteins and Other Molecules > Protein–RNA Interactions: Functional Implications RNA Interactions with Proteins and Other Molecules > Protein–RNA Recognition RNA Interactions with Proteins and Other Molecules > RNA–Protein Complexes RNA Evolution and Genomics > Ribonomics