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RNA structure, binding, and coordination in Arabidopsis
Author(s) -
Foley Shawn W.,
Kramer Marianne C.,
Gregory Brian D.
Publication year - 2017
Publication title -
wiley interdisciplinary reviews: rna
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.225
H-Index - 71
eISSN - 1757-7012
pISSN - 1757-7004
DOI - 10.1002/wrna.1426
Subject(s) - rna , computational biology , riboswitch , nucleic acid secondary structure , rna binding protein , rna induced transcriptional silencing , protein secondary structure , transcription (linguistics) , arabidopsis , biology , non coding rna , genetics , gene , biochemistry , mutant , linguistics , philosophy
From the moment of transcription, up through degradation, each RNA transcript is bound by an ever‐changing cohort of RNA binding proteins. The binding of these proteins is regulated by both the primary RNA sequence, as well as the intramolecular RNA folding, or secondary structure, of the transcript. Thus, RNA secondary structure regulates many post‐transcriptional processes. With the advent of next generation sequencing, several techniques have been developed to generate global landscapes of both RNA –protein interactions and RNA secondary structure. In this review, we describe the current state of the field detailing techniques to globally interrogate RNA secondary structure and/or RNA –protein interaction sites, as well as our current understanding of these features in the transcriptome of the model plant Arabidopsis thaliana . WIREs RNA 2017, 8:e1426. doi: 10.1002/wrna.1426 This article is categorized under: RNA Structure and Dynamics > RNA Structure, Dynamics, and Chemistry RNA Interactions with Proteins and Other Molecules > Protein–RNA Recognition