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Molecular diagnostics for personal medicine using a nanopore
Author(s) -
Mirsaidov Utkur M.,
Wang Deqiang,
Timp Winston,
Timp Gregory
Publication year - 2010
Publication title -
wiley interdisciplinary reviews: nanomedicine and nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 72
eISSN - 1939-0041
pISSN - 1939-5116
DOI - 10.1002/wnan.86
Subject(s) - nanopore sequencing , nanopore , nanotechnology , dna , computational biology , sensitivity (control systems) , computer science , polymerase chain reaction , dna sequencing , biophysics , materials science , biology , genetics , gene , engineering , electronic engineering
Semiconductor nanotechnology has created the ultimate analytical tool: a nanopore with single molecule sensitivity. This tool offers the intriguing possibility of high‐throughput, low cost sequencing of DNA with the absolute minimum of material and preprocessing. The exquisite single molecule sensitivity obviates the need for costly and error‐prone procedures like polymerase chain reaction amplification. Instead, nanopore sequencing relies on the electric signal that develops when a DNA molecule translocates through a pore in a membrane. If each base pair has a characteristic electrical signature, then ostensibly a pore could be used to analyze the sequence by reporting all of the signatures in a single read without resorting to multiple DNA copies. The potential for a long read length combined with high translocation velocity should make resequencing inexpensive and allow for haplotyping and methylation profiling in a chromosome. WIREs Nanomed Nanobiotechnol 2010 2 367–381 This article is categorized under: Diagnostic Tools > Diagnostic Nanodevices