Premium
Temporal flickering of contrast agents for enhanced optical imaging
Author(s) -
Ilovitsh Tali,
Zalevsky Zeev
Publication year - 2015
Publication title -
wiley interdisciplinary reviews: nanomedicine and nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 72
eISSN - 1939-0041
pISSN - 1939-5116
DOI - 10.1002/wnan.1375
Subject(s) - flicker , contrast (vision) , computer science , microscopy , modality (human–computer interaction) , artificial intelligence , computer vision , optics , biological system , physics , computer graphics (images) , biology
The temporal flickering of contrast agents that labels a biological sample is a unique modality for cellular imaging with single molecule sensitivity. It improves the signal‐to‐noise ratio statistics associated with the noisy in vivo environment and has promising applications in single particle tracking and super‐resolution microscopy techniques. The flickering can be triggered either statistically through the mechanism of temporal fluctuations of the emitter or through external modulation. The enriching toolbox of contrast agents that are feasible for biomedical imaging for the flickering methods will be discussed, with emphasis on the emerging field of flickering gold nanoparticles and the lock‐in detection mechanism. WIREs Nanomed Nanobiotechnol 2016, 8:439–448. doi: 10.1002/wnan.1375 This article is categorized under: Diagnostic Tools > In Vitro Nanoparticle-Based Sensing Diagnostic Tools > In Vivo Nanodiagnostics and Imaging