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Protein nanomachines assembly modes: cell‐free expression and biochip perspectives
Author(s) -
Daube Shirley S.,
BarZiv Roy H.
Publication year - 2013
Publication title -
wiley interdisciplinary reviews: nanomedicine and nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 72
eISSN - 1939-0041
pISSN - 1939-5116
DOI - 10.1002/wnan.1234
Subject(s) - biochip , nanotechnology , synthetic biology , ribosome , microbiology and biotechnology , biology , computational biology , template , cytoplasm , biophysics , rna , biochemistry , materials science , gene
Large macromolecular assemblies are widespread in all cell types with diverse structural and functional roles. Whether localized to membranes, nuclei, or cytoplasm, multimeric protein–nucleic acid complexes may be viewed as sophisticated nanomachines, an inspiration to chemical design. The formation of large biological assemblies follows a complex and hierarchical self‐assembly process via ordered molecular recognition events. Serving a paradigm for biological assembly, extensive past studies of T4 bacteriophage and bacterial ribosomes by many groups have been revealing distinct design strategies, yet these two very different multimeric complexes share common mechanistic motifs. An emerging biochip approach highlights two conceptual notions to promote the study of assembly pathways: cell‐free expression provides coupling between synthesis and assembly; surface anchoring allows high‐resolution imaging of structural intermediates and opens up opportunities for rewiring a network by defining unnatural scaffolds for synthetic design applications. WIREs Nanomed Nanobiotechnol 2013, 5:613–628. doi: 10.1002/wnan.1227 This article is categorized under: Nanotechnology Approaches to Biology > Nanoscale Systems in Biology