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Surface‐enhanced Raman scattering imaging using noble metal nanoparticles
Author(s) -
Wilson Andrew J.,
Willets Katherine A.
Publication year - 2013
Publication title -
wiley interdisciplinary reviews: nanomedicine and nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 72
eISSN - 1939-0041
pISSN - 1939-5116
DOI - 10.1002/wnan.1208
Subject(s) - raman scattering , nanotechnology , materials science , nanoparticle , colloidal gold , raman spectroscopy , nanoscopic scale , preclinical imaging , molecular imaging , in vivo , optics , physics , microbiology and biotechnology , biology
Surface‐enhanced Raman scattering (SERS) imaging is a powerful technique for studying biological systems both in vitro and in vivo . In SERS, Raman scattering from molecules located near the surface of either gold or silver nanoparticles is enhanced by 10 5 –10 8 . This review describes the basic enhancement mechanism of SERS and provides experimental details that must be considered when performing a SERS imaging experiment with a focus on cellular imaging. Specific examples highlighting the power of SERS for measuring chemical distributions in cells, signal multiplexing, and following dynamic motion of SERS probes in vivo are provided. Potential future directions in which SERS is combined with super‐resolution imaging are also described. WIREs Nanomed Nanobiotechnol 2013, 5:180–189. doi: 10.1002/wnan.1208 This article is categorized under: Diagnostic Tools > Diagnostic Nanodevices Diagnostic Tools > In Vitro Nanoparticle-Based Sensing Nanotechnology Approaches to Biology > Nanoscale Systems in Biology