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Multiphoton microscopy applications in nanodermatology
Author(s) -
Prow Tarl W.
Publication year - 2012
Publication title -
wiley interdisciplinary reviews: nanomedicine and nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 72
eISSN - 1939-0041
pISSN - 1939-5116
DOI - 10.1002/wnan.1189
Subject(s) - microscopy , nanoparticle , nanotechnology , nanomedicine , materials science , quantum dot , fluorescence , femtosecond , fluorescence microscope , two photon excitation microscopy , confocal microscopy , fluorescence lifetime imaging microscopy , confocal , nanotoxicology , laser , optics , physics
Multiphoton microscopy is being used as a tool for tracking nanoparticles and assessing endogenous autofluorescent molecules. This technology is founded on femtosecond lasers that are capable of exciting unlabeled metal nanoparticles, quantum dots, and upconverting nanoparticles. The addition of time‐correlated single‐photon counting detectors enables fluorescence lifetime imaging. Fluorescence lifetime measurements result in high‐resolution, quantitative data that can be used to distinguish nanoparticle signals from those of endogenous fluorophores. This application of multiphoton microscopy is capable of simultaneous nanoparticle and NAD(P)H imaging, resulting in the capacity for dye‐free assessments of treatment‐induced metabolic changes. The stage is set for advanced, clinical imaging focused nanoparticle trials that can directly address critical issues in nanomedicine and nanotoxicology. WIREs Nanomed Nanobiotechnol 2012, 4:680–690. doi: 10.1002/wnan.1189 This article is categorized under: Diagnostic Tools > In Vivo Nanodiagnostics and Imaging Toxicology and Regulatory Issues in Nanomedicine > Toxicology of Nanomaterials