Open AccessFaecal proteome in clinically healthy dogs and cats: Findings in pooled faeces from 10 cats and 10 dogs
Author(s) -
Cerquetella Matteo,
Marchegiani Andrea,
Mangiaterra Sara,
Rossi Giacomo,
Gavazza Alessandra,
Tesei Beniamino,
Spaterna Andrea,
Sagratini Gianni,
Ricciutelli Massimo,
Polzonetti Valeria,
Pucciarelli Stefania,
Vincenzetti Silvia
Publication year - 2021
Publication title -
veterinary record open
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.504
H-Index - 12
eISSN - 2052-6113
pISSN - 2399-2050
DOI - 10.1002/vro2.9
Subject(s) - cats , feces , albumin , proteome , biology , proteomics , alkaline phosphatase , transthyretin , pathology , microbiology and biotechnology , biochemistry , medicine , chemistry , endocrinology , enzyme , gene
Abstract Background In the scientific literature, there are only a few manuscripts available on small animal faecal proteomics. Methods In the present pilot study, this evaluation was performed using pooled faecal samples from 10 clinically healthy dogs and, for the first time, in 10 clinically healthy cats by mean of two‐dimensional electrophoresis followed by liquid chromatography‐tandem mass spectrometry. Results Our results showed the presence of nine (albumin, alkaline phosphatase, chymotrypsin‐C‐like, cytosol aminopeptidase, elastase‐3B/proteinase E, immunoglobulins and nuclear pore membrane glycoprotein 210) and 14 (albumin, caspase recruitment domain‐containing protein, chymotrypsin‐like, deleted in malignant brain tumours 1 protein‐like, hypothetical protein LOC107375, immunoglobulin, kallikrein‐1, superoxide dismutase, transthyretin precursor, interstitial collagenase‐like) different proteins in canine and feline faeces, respectively. Conclusion These preliminary findings document the presence of a range of proteins in the faeces of apparently healthy dogs and cats and may serve as a basis for larger, prospective studies to establish reference proteomic data against which diseased populations can be compared.