Open AccessPrevalence of Anaplasmataceae and Filariidae species in unowned and military dogs in New Caledonia
Author(s) -
Dahmani Mustapha,
Tahir Djamel,
Cabre Olivier,
Raoult Didier,
Fenollar Florence,
Davoust Bernard,
Mediannikov Oleg
Publication year - 2018
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.97
Subject(s) - anaplasmataceae , wolbachia , biology , dirofilaria immitis , anaplasma , zoology , microbiology and biotechnology , tick , virology , ecology , host (biology) , helminths
Dogs are competent reservoir hosts of several zoonotic agents, including Filariidae nematodes and Anaplasmataceae family bacteria. The latter family unites human and veterinary pathogens ( Anaplasma, Ehrlichia and Neorickettsia bacteria) with Wolbachia , some of which are obligatory endosymbionts of pathogenic filarial nematodes. The epidemiology of Anaplasmataceae and Filariidae species infecting dogs living in kennels in New Caledonia was studied. 64 EDTA blood samples were screened for the presence of Anaplasmataceae and filarial nematodes. Molecular study was conducted using primers and probe targeting the of 23S rRNA long fragment of Anaplasmataceae species. Next, all blood sample was screened for the presence of Filariidae species targeting the primers and probe targeting the COI gene, as well as primers targeting the COI and 5S rRNA genes of all filarial worms. Anaplasma platys was identified in 8/64 (12.5, 95% confidence interval [ CI ]: 4.4–20.6%) and Wolbachia endosymbiont of Dirofilaria immitis in 8/64 (12.5%, CI : 4.4–20.6%). Filariidae species investigation was performed and showed that 11/64 (17.2%, CI : 7.9–26.4%) dogs were infected with D . immitis , whereas, 2/64 (3.1%, CI : 0.0–7.3%) were infected with Acanthocheilonema reconditum . Finally, we checked the occurrence of co‐infection between Anaplasmataceae and Filariidae species. Co‐occurrence with Wolbachia endosymbiont of D . immitis was observed in seven dogs, one dog was co‐infected with A . platys and A . reconditum and another was co‐infected with Wolbachia endosymbiont of D . immitis and A . reconditum . These results are the first report of Anaplasmataceae and Filariidae occurring in dogs in New Caledonia.