Open AccessAnti‐inflammatory effect of mesenchymal stem cells on hepatocellular carcinoma in the xenograft mice model
Author(s) -
Hajighasemlou Saieh,
Nikbakht Mohsen,
Pakzad Saeedreza,
Azadbakht Abdolnaser,
Muhammadnejad Samad,
Mirmoghtadaei Milad,
Gharibzadeh Safoora,
Seyhoun Iman,
Verdi Javad
Publication year - 2022
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.886
Subject(s) - mesenchymal stem cell , hepatocellular carcinoma , sorafenib , medicine , aspartate transaminase , alanine transaminase , liver cancer , inflammation , cancer research , cancer , tumor necrosis factor alpha , necrosis , carcinoma , pathology , biology , alkaline phosphatase , enzyme , biochemistry
Background Hepatocellular carcinoma (HCC) is the fifth most diagnosed cancer and the second leading cause of cancer‐related deaths worldwide. Sorafenib is the standard treatment used in the advanced stages of HCC. Cell therapy with mesenchymal stem cells (MSCs)‐based cell therapy has proven effective in immune regulation and tumour growth inhibition. Objectives In this study, we investigated the anti‐inflammatory effect of MSCs on HCC xenografts. Methods Human HepG2 cell lines were subcutaneously implanted into the flank of 12 nude mice, divided into three groups: the control group, the IV group (intravenous MSCs injection) and the local group (local MSCs injection). Mice were sacrificed 6 weeks after tumour implantation, and tumours were resected entirety. Quantitative real‐time polymerase chain reaction (qRT‐PCR) measured the gene expression of inflammatory markers, including tumour necrosis factor‐α (TNF‐α), interleukin (IL)‐1α and IL‐10. Aspartate transaminase (AST), alanine transaminase (ALT) and urea levels were measured using spectrophotometry to ensure the safety of MSC therapy. Results Gene expressions for all three inflammatory markers were reduced in both MSCs groups compared to the control group. AST, ALT and urea levels remained in normal ranges. Conclusions MSC therapy can reduce inflammation in HCC xenograft mouse models.