Open AccessOutbreak of porcine epidemic diarrhoea virus ( PEDV ) in Abruzzi region, central‐Italy
Author(s) -
Pizzurro Federica,
Cito Francesca,
Zaccaria Guendalina,
Spedicato Massimo,
Cerella Angelo,
Orsini Massimiliano,
Forzan Mario,
D'Alterio Nicola,
Lorusso Alessio,
Marcacci Maurilia
Publication year - 2018
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.88
Subject(s) - porcine epidemic diarrhea virus , outbreak , virology , feces , biology , indel , whole genome sequencing , genome , virus , diarrhea , genotype , microbiology and biotechnology , genetics , gene , single nucleotide polymorphism , medicine
Here we report and characterize a porcine epidemic diarrhea (PED) outbreak which occurred in a swine fattening farm in the province of Teramo, Abruzzi region (central Italy), in January 2016. PED virus (PEDV) identification was determined by real‐time RT‐PCR performed on RNAs purified from fecal samples collected from two symptomatic pigs. Whole genome sequence (PEDV 1842/2016) was also obtained by next generation sequencing straight from RNA purified from one fecal sample. Genome comparison with extant global PEDV strains revealed a high nucleotide identity with recently reported European and American S‐INDEL PEDVs. Efficient sequencing, share of genomic data combined with the implementation of epidemiological tools would be the ideal approach for study and analysis of transboundary infectious diseases as PED .