Open AccessPhylogenetic analysis of porcine circovirus 3 circulating in Canadian pigs
Author(s) -
Zhang Maodong,
Liu Chao Chun,
Huang Yanyun,
Hill Janet E.,
Bravo Araya Maria,
Ojkic Davor,
Gag Carl A.
Publication year - 2022
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.851
Subject(s) - porcine circovirus , phylogenetic tree , antigenicity , circovirus , capsid , biology , virology , pathogenicity , genetic diversity , phylogenetic relationship , genetics , gene , microbiology and biotechnology , virus , antibody , medicine , population , environmental health
Abstract Introduction Porcine circovirus 3 (PCV3) has been detected in pigs worldwide and associated with several clinical signs. Methods To investigate the genetic diversity of PCV3 strains circulating in Canada, 44 PCV3 positive samples from Saskatchewan (2/44), Manitoba (2/44), Quebec (4/44), Alberta (11/44) and Ontario (25/44) submitted to diagnostic laboratories in Canada between 2019 and 2021 were sequenced and analyzed. Results Phylogenetic analysis of capsid genes showed that all of the 44 Canadian strains classified into PCV3a and segregated into seven lineages with common amino acid changes observed at A24V, R27K, N56D, T77S, Q98R, L150I (F) and R168K positions. Conclusion Future studies are required to determine whether the polymorphisms in capsid proteins, as revealed in this study, could be associated with differences in the pathogenicity or antigenicity of PCV3 strains. This is the first phylogenetic analysis of PCV3 strains among different provinces in Canada.