Open AccessThe effect of sodium bicarbonate and validation of beckman coulter AU 680 analyzers for measuring total carbon dioxide ( TC O 2 ) concentrations in horse serum
Author(s) -
Dirikolu Levent,
Waller Pamela,
Waguespack Mona Landry,
Andrews Frank Michael,
Keowen Michael Layne,
Gaunt Stephen David
Publication year - 2017
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.82
Subject(s) - sodium bicarbonate , bicarbonate , sodium , confidence interval , horse , spectrum analyzer , chemistry , carbon dioxide , zoology , chromatography , analytical chemistry (journal) , medicine , physics , biology , paleontology , organic chemistry , optics
This study evaluated the usage of Beckman Coulter AU 680 analyzers for measurement of TCO 2 in horse serum, and the effect of sodium bicarbonate administrations on serum TCO 2 levels in resting horses. Treatment of horses with sodium bicarbonate did not result in any adverse events. Mean TCO 2 concentration was significantly higher from 1 to 8 h in the sodium bicarbonate‐treated horses compared to the untreated controls. Within an hour, administration of sodium bicarbonate increased the TCO 2 level from 31.5 ± −2.5 ( SD ) to 34.0 ± 2.65 ( SD ) mmol/L and at 2–8 h post‐administration, the TCO 2 level was above the 36 mmol/L cut‐off level. In all quality control analysis of Australian standard by Beckman Coulter AU 680 analyzer, the instrument slightly over estimated the TCO 2 level but the values were in close agreement with mean TCO 2 level being 38.03 with ± 0.87 mmol/L ( SD ). Expanded uncertainty was calculated using different levels of confidence interval. Based on 99.5% confidence interval using 0.805% expanded uncertainty using mean measured concentration of 38.05 mmol/L, it was estimated that any race samples TCO 2 level higher than 38.5 mmol/L will be indicative of sodium bicarbonate administration using Beckman Coulter AU 680 analyzer in Louisiana.