Open AccessMolecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia
Author(s) -
Sallemi Souhir,
Rjeibi Mohamed R.,
Rouatbi Mariem,
Amairia Safa,
Ben Said Mourad,
Khamassi Khbou Madiha,
Gharbi Mohamed
Publication year - 2018
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.79
Subject(s) - trypanosoma evansi , veterinary medicine , phylogenetic tree , biology , theileria , virology , trypanosomiasis , gene , parasite hosting , medicine , genetics , world wide web , computer science
The present study aimed to estimate the molecular prevalence of Theileria annulata and Trypanosoma evansi infection in cattle in Northern Tunisia. A total number of 96 cattle from five farms were evaluated. T. annulata and T. evansi prevalences were 61% [56/66] and 10% [7/13], respectively, at a confidence interval ( CI ) of 95%, while co‐infection was present in 6% [4/8] of the tested animals at a CI of 95%. There was a significant correlation between age and the prevalence of T. annulata infection, whereas, there was no significant association shown with the age of cattle and T. evansi infection. Sequence and phylogenetic analyses showed that the T. annulata Tams1 gene and T. evansi ITS 1 rDNA gene were highly conserved with 97.1–100% and 98.3–100% sequence identity, respectively.