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The present study aimed to estimate the molecular prevalence of  Theileria annulata  and  Trypanosoma evansi  infection in cattle in Northern Tunisia. A total number of 96 cattle from five farms were evaluated.  T. annulata  and  T. evansi  prevalences were 61% [56/66] and 10% [7/13], respectively, at a confidence interval ( CI ) of 95%, while co‐infection was present in 6% [4/8] of the tested animals at a  CI  of 95%. There was a significant correlation between age and the prevalence of  T. annulata  infection, whereas, there was no significant association shown with the age of cattle and  T. evansi  infection. Sequence and phylogenetic analyses showed that the  T. annulata  Tams1 gene and  T. evansi   ITS 1  rDNA  gene were highly conserved with 97.1–100% and 98.3–100% sequence identity, respectively.

Molecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia