Open AccessMolecular evaluation and genetic characterisation of Newcastle disease virus's haemagglutinin‐neuraminidase protein isolated from broiler chickens in Iran
Author(s) -
Shafaati Mohammadreza,
Ghorbani Masoud,
Mahmodi Minoo,
Ebadi Mostafa,
Jalalirad Reza
Publication year - 2022
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.629
Subject(s) - newcastle disease , biology , virology , phylogenetic tree , neuraminidase , virus , gene , genotype , sequence analysis , outbreak , molecular epidemiology , broiler , genetics , zoology
Background Newcastle disease (ND) virus (NDV) is one of the major pathogens in poultry farms that causes severe economic damages to the poultry industry, especially broiler chicken and turkey farms. Despite the endemicity of ND and its many epidemics in the country, the nature of the Iranian strain of the Newcastle virus is still largely unknown. This study aimed to characterise and evaluate NDV isolates obtained from commercial poultry farms in Iran in 2019 through haemagglutinin‐neuraminidase (HN) gene sequencing. Method HN gene of each NDV isolate was amplified and sequenced using specific primers followed by phylogenetic analysis of full length of HN gene open reading frame and amino acid (aa) sequence of HN. Results Phylogenetic analysis of the HN gene showed that the virus is very closely related to genotypes VII and III. Analysis of HN gene nucleotide sequences showed that all isolates encode proteins with a length of 571 aa. Conclusion Results of the present study are useful for a better understanding of molecular epidemiology of indigenous NDV strains and determining important molecular differences between fields and commonly used vaccine strains related to main immunogenic proteins.