Open Access“Immunolocalization and effect of low concentrations of Insulin like growth factor‐1 (IGF‐1) in the canine ovary”
Author(s) -
Cardilli Diogo J.,
SousaOliveira Kellen,
FranchiJoão Carolina,
AzevedoVoorwald Faviana,
MachadoSilva Marco A.,
Oliveira João Ademir,
SánchezCalabuig María Jesús,
Toniollo Gilson H.,
PérezGutiérrez José F.
Publication year - 2021
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.347
Subject(s) - corpus luteum , ovary , insulin like growth factor , oocyte , endocrinology , medicine , follicular phase , growth factor , biology , immunohistochemistry , follicle , chemistry , receptor , andrology , microbiology and biotechnology , embryo
Insulin like growth factor‐1 (IGF‐1) plays an important role in the regulation of ovarian function. Despite its extensive study in several species, there is a paucity of information about IGF‐1`s function and localization in the canine ovary. The aim of the present study was to assess the effect of IGF‐1 on oocyte nuclear maturation and to immunolocalize the IGF‐1 and its receptor (IGF‐1R) in the ovary. Cumulus‐oocyte complexes (COCs) were obtained from 34 bitches. The COCs from each bitch were incubated in TCM 199‐HEPES in the absence ( n = 199) or presence ( n = 204) of 100 ng/ml IGF‐1 for 96 hr at 38ºC in 5% CO 2 , stained and evaluated for nuclear maturation by fluorescence microscopy. The results showed that the addition of IGF‐1 did not have an effect ( p ˃ 0.05) on the nuclear maturation under these conditions. The immunohistochemical study revealed nuclear and cytoplasmic staining for IGF‐1 and IGF‐1R, respectively. Both were localized in all ovarian structures including the corpus luteum, but not in the granulosa cells from primordial follicles. In addition, IGF‐1 was not localized in the oocytes in tertiary follicles. The results obtained show the presence of IGF‐1 through the stages of follicular growth and in the corpus luteum of the canine ovary. However, its role on oocyte nuclear maturation could not be demonstrated.