Open AccessTrace amounts of African swine fever virus DNA detected in insects collected from an infected pig farm in Estonia
Author(s) -
Herm Reet,
Tummeleht Lea,
Jürison Margret,
Vilem Annika,
Viltrop Arvo
Publication year - 2020
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.200
Subject(s) - outbreak , african swine fever virus , biology , transmission (telecommunications) , dna virus , veterinary medicine , virus , virology , insect , vector (molecular biology) , isolation (microbiology) , mechanical transmission , zoology , ecology , microbiology and biotechnology , medicine , recombinant dna , biochemistry , mechanical system , genome , artificial intelligence , gene , computer science , electrical engineering , engineering
Background African swine fever (ASF), a severe multi‐systemic disease in pigs, was introduced into Estonia in 2014. The majority of outbreaks have occurred during the summer months. Given that ASFV is transmitted in a sylvatic cycle that includes the transmission by African soft ticks and that mechanical transmission by flying insects was shown, transmission by other arthropod vectors need to be considered. Objectives Here, we report the results of a pilot study on flying insects caught on an outbreak farm during epidemiological investigations. Methods In brief, 15 different insect species (flies and mosquitoes) were collected by random catch using an aerial net. Nucleic acids derived from these samples or their pools were tested for African swine fever virus (ASFV) DNA by real‐time PCR. Results and Conclusions Viral DNA was detected in small quantities in two samples from flies and mosquitoes. Given the slow spread of virus within the farm, the impact of these findings seems rather low, but a role in local transmission cannot be ruled out. However, given the very low number of insects sampled, and taken into the account that viral isolation was not performed and insects outside the farm were not investigated, future investigations are needed to assess the true impact of insects as mechanical vectors.