Open AccessFirst isolation, identification and genetic characterization of Brucella abortus biovar 3 from dairy cattle in Bangladesh
Author(s) -
Islam Md. Sadequl,
Garofolo Giuliano,
Sacchini Lorena,
Dainty Amanda C.,
Khatun Mst. Minara,
Saha Sukumar,
Islam Md. Ariful
Publication year - 2019
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.193
Subject(s) - multiple loci vntr analysis , biovar , brucella , biology , brucellosis , veterinary medicine , microbiology and biotechnology , variable number tandem repeat , genotype , brucellaceae , isolation (microbiology) , brucella abortus , bacteria , virology , brucella melitensis , genetics , gene , medicine
Background Brucellosis is a zoonotic disease caused by bacteria Brucella spp. belonging to the genus Brucella . It is endemic in domesticated animals in Bangladesh. Isolation, identification and genetic characterization of Brucella spp. in dairy cattle are essential to undertake appropriate control and preventive measures. The study was conducted to isolate and characterize the Brucella spp. circulating in dairy cattle. Methods Uterine discharge ( n = 45), milk ( n = 115), vaginal swab ( n = 71), placenta ( n = 7) and aborted fetus ( n = 2) were collected. Brucella selective agar plates were inoculated with samples and incubated at 37 ◦ C for 14 days under 5% CO 2 for isolation of Brucella spp. Brucella suspected colonies were recovered from samples were confirmed by genus and species specific PCR assays. Genetic characterization was performed by Multi Locus Variable number tandem‐repeat Analysis‐16 (MLVA‐16). Results The isolates of Brucella recovered from samples were confirmed as B. abortus by AMOS‐ERY PCR assay. The classical biotyping method confirmed all 10 B. abortus isolates belonged to the biovar 3. The MLVA‐16 assay indicated all B. abortus isolates identical and the same genotype 40, based on panel 1 MLVA‐8. Conclusion Dendrogram analysis revealed all B. abortus isolates of the study were identical to three isolates from Brazil, one isolate of France and closely related to Chinese isolates. This is the first report of isolation and genetic characterization of B. abortus from the dairy cattle in Bangladesh.