Open AccessEstablishment of a sensitive TaqMan‐based real‐time PCR assay for porcine circovirus type 3 and its application in retrospective quarantine of imported boars to China
Author(s) -
Feng Chunyan,
Wang CaiXia,
Zhang Yongning,
Du Fangyuan,
Zhang Zhou,
Xiao Fuchuan,
Wang Jianchang,
Lin Xiangmei,
Wu Shaoqiang
Publication year - 2019
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.141
Subject(s) - porcine circovirus , taqman , virology , quarantine , biology , circovirus , real time polymerase chain reaction , plasmid , outbreak , veterinary medicine , virus , dna , gene , medicine , genetics , ecology
Porcine circovirus type 3 ( PCV 3) is a novel pathogen first identified in the United States in 2016. As there is a high possibility that no clinical signs of infection are observed in the host, an accurate and sensitive method is needed for quarantine on numerous live pigs especially for international pig trade. In this study, a TaqMan‐based real‐time PCR assay specifically for PCV 3 was established without cross‐reactions with other non‐targeted pig viruses. The sensitivity of the current approach is about 1.5 × 10 1 copies μ L −1 plasmid DNA while the sensitivity of the conventional PCR is about 1.5 × 10 2 copies μ L −1 plasmid DNA . Further, this assay was applied in the retrospective quarantine on serum samples of 601 commercial live boars imported to China from the United States, France and the United Kingdom from 2011 to 2017. The results revealed that PCV 3 could be detected positive in the commercial boars imported from the United States and the above‐mentioned western European countries and phylogenetic study also revealed that viral isolates were grouped with some isolates from Korea and the United States. Our study suggested that PCV 3 may be prevalent globally since 2011.