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The application of ion‐pair high performance countercurrent chromatography monitored by off‐line LC‐ESI‐MS/MS injections to study betalain metabolite from Vietnamese red dragon fruit ( Hylocereus polyrhizus )
Author(s) -
Thu Tran Thi Minh,
Jerz Gerold,
Winterhalter Peter
Publication year - 2019
Publication title -
vietnam journal of chemistry
Language(s) - English
Resource type - Journals
eISSN - 2572-8288
pISSN - 0866-7144
DOI - 10.1002/vjch.201900049
Subject(s) - chemistry , chromatography , betanin , trifluoroacetic acid , betalain , pigment , organic chemistry
Abstract The pre‐purified betalain extract from Vietnamese red dragon fruits ( Hylocereus polyrhizus ) was fractionated by a high performance countercurrent chromatography (HPCCC) device using the ion‐pair forming biphasic solvent of tert ‐butylmethylether/ n ‐butanol/acetonitrile/1% aqueous trifluoroacetic acid (2:2:1:5, v/v/v/v). The J ‐type HPCCC centrifuge with a 240g centrifugal force field was able to fractionate seven pigments from a 350 mg enriched crude pigment fruit extract, resulting in purified fractions for qualitative identification by LC‐ESI‐MS/MS. This approach fractionated the main betacyanin epimers namely 15R/15S‐ betanin ( m/z 551), 15R/15S‐ phyllocactin ( m/z 637), and apiosyl‐ 15R/15S‐ betanin ( m/z 683) from the solely existing betaxanthin indicaxanthin. In addition, few minor betacyanins such as 2’‐apiosyl‐ 15R/15S‐ phyllocactin ( m/z 769) were concentrated in a few fractions. That method also allowed the better detection of cacti fruit pigment 15R/15S‐ hylocerenin ( m/z 695), and 15R/15S‐ betanidin 5‐O‐ β ‐sophoroside ( m/z 713). However, the separation of these highly polar compounds was incomplete due to insufficient force of the ion‐pairing effect of trifluoroacetic acid (TFA).