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Anti‐inflammatory and cytotoxic activities of constituents from Wedelia trilobata (L.) Hitchc.
Author(s) -
Thao Nguyen Phuong,
Binh Pham Thanh,
Luyen Nguyen Thi,
Cong Nguyen Duy,
Dang Nguyen Hai,
Dat Nguyen Tien
Publication year - 2019
Publication title -
vietnam journal of chemistry
Language(s) - English
Resource type - Journals
eISSN - 2572-8288
pISSN - 0866-7144
DOI - 10.1002/vjch.201900001
Subject(s) - apigenin , chemistry , cinnamic acid , two dimensional nuclear magnetic resonance spectroscopy , cytotoxic t cell , oleanolic acid , methanol , stereochemistry , traditional medicine , organic chemistry , biochemistry , flavonoid , in vitro , antioxidant , medicine , alternative medicine , pathology
Using various chromatographic separations, five compounds ( 1 – 5 ) were isolated from a methanol extract of W. trilobata leaves. Their structures were elucidated to be paniculoside‐IV ( 1 ), 3‐ O ‐[ β ‐D‐glucopyranosyl(1‐4)‐ β ‐D‐glucoronopyranosyl] oleanolic acid 28‐ O ‐ β ‐D‐glucopyranosyl ester ( 2 ), 5,7,4'‐trihydroxyflavone ( 3 ), apigenin 7‐ O ‐ β ‐D‐glucopyranoside ( 4 ), and 3,4‐dihydroxy‐cinnamic acid ( 5 ), by detailed analysis via spectroscopic techniques (IR, 1D, 2D NMR and ESI‐MS data) as well as comparison with those reported. This is the first report of 2 from Wedelia genus. Furthermore, the effects of the isolated compounds on LPS‐induced NO production in RAW264.7 cells and cytotoxic activity (against A549, MCF‐7, Hep3B, and PC3 cell lines) were also evaluated.