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Ultrasound increases DNA damage attributable to cisplatin in cisplatin‐resistant human ovarian cancer cells
Author(s) -
Yu T.,
Yang Y.,
Liu S.,
Yu H.
Publication year - 2009
Publication title -
ultrasound in obstetrics and gynecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.202
H-Index - 141
eISSN - 1469-0705
pISSN - 0960-7692
DOI - 10.1002/uog.6258
Subject(s) - cisplatin , comet assay , dna damage , ovarian cancer , medicine , chemotherapy , dna , ultrasound , cancer research , cancer , microbiology and biotechnology , biology , genetics , radiology
Objective An increased capacity for DNA repair plays a very important role in cisplatin (DDP) resistance in ovarian cancers. Ultrasound is a potential chemotherapy sensitizer. The aim of this study was to determine whether ultrasound in conjunction with chemotherapy increases DNA damage in chemoresistant human ovarian cancer cells. Methods Ultrasound and/or cyclosporin A were used to overcome chemoresistance in a DDP‐resistant human ovarian cancer cell line, COC1/DDP. DNA damage was quantified by comet assay, a form of single‐cell gel electrophoresis in which the length of the comet tail reflects the level of DNA damage. Results Neither ultrasound nor cyclosporin A alone led to detectable DNA breakage. The use of ultrasound increased DNA breakage due to DDP, while the use of cyclosporin A did not. The addition of ultrasound and cyclosporin A in conjunction with DDP resulted in a 2.55 times increase in the length of comet tail compared with using DDP alone, while their combined use resulted in a 1.73 times increase compared with the combination of just DDP and insonation. Conclusions Insonation increases DNA breakage attributable to DDP in chemoresistant human ovarian cancer cells, and might sensitize cyclosporin A. Copyright © 2009 ISUOG. Published by John Wiley & Sons, Ltd.