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EP21.01: Confocal microtomography (micro‐CT) analysis and three‐dimensional (3D) reconstruction of pathological conditions affecting the human fallopian tube (FT)
Author(s) -
Castro P.T.,
Aranda O.L.,
Matos A.P.,
Marchiori E.,
Alves H.D.,
Lopes R.T.,
Machado A.S.,
Werner H.,
Araujo Junior E.
Publication year - 2018
Publication title -
ultrasound in obstetrics and gynecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.202
H-Index - 141
eISSN - 1469-0705
pISSN - 0960-7692
DOI - 10.1002/uog.20074
Subject(s) - medicine , fallopian tube , microscopy , confocal microscopy , pathological , tomography , biomedical engineering , virtual microscopy , confocal , radiology , pathology , anatomy , biology , optics , physics , microbiology and biotechnology
Methods: Eight specimens of FT affected by pathological conditions, from elective and emergency surgeries were selected, three Ectopic pregnancies (EP) from emergency surgeries and five remnants of FT (RFT) from surgical sterilization. The specimens were fixed in formalin for 24h and stained in Lugol solution for 72h. The micro-CT studies were conducted using protocols adapted from biological studies and the specimens were evaluated by traditional microscopy. Conclusion: Micro-CT is able to demonstrate many previously described characteristics of pathological FT in 3D, with good tissue contrast and can help guiding traditional histopathological sectioning of specimens.